GlutaredoxinV1, Main, Exploration, bibRecord, 000483

1H, 13C and 15N resonance assignment of the cytosolic dithiol glutaredoxin 1 from the pathogen Trypanosoma brucei.

Identifieur interne : 000483 ( Main/Exploration ); précédent : 000482; suivant : 000484

1H, 13C and 15N resonance assignment of the cytosolic dithiol glutaredoxin 1 from the pathogen Trypanosoma brucei.

Auteurs : Monica Stefani [Italie] ; Mattia Sturlese [Italie] ; Bruno Manta [Uruguay] ; Frank Löhr [Allemagne] ; Stefano Mammi [Italie] ; Marcelo Comini [Uruguay] ; Massimo Bellanda [Italie]

Source :

Biomolecular NMR assignments [ 1874-270X ] ; 2016.

RBID : pubmed:26386962

Descripteurs français

KwdFr :
- Cytosol (métabolisme), Domaine catalytique (MeSH), Glutarédoxines (composition chimique), Humains (MeSH), Isotopes de l'azote (MeSH), Isotopes du carbone (MeSH), Résonance magnétique nucléaire biomoléculaire (MeSH), Structure secondaire des protéines (MeSH), Séquence d'acides aminés (MeSH), Toluène (analogues et dérivés), Toluène (composition chimique), Toluène (métabolisme), Tritium (MeSH), Trypanosoma brucei brucei (métabolisme).
MESH :
- analogues et dérivés : Toluène.
- composition chimique : Glutarédoxines, Toluène.
- métabolisme : Cytosol, Toluène, Trypanosoma brucei brucei.
- Domaine catalytique, Humains, Isotopes de l'azote, Isotopes du carbone, Résonance magnétique nucléaire biomoléculaire, Structure secondaire des protéines, Séquence d'acides aminés, Tritium.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Carbon Isotopes (MeSH), Catalytic Domain (MeSH), Cytosol (metabolism), Glutaredoxins (chemistry), Humans (MeSH), Nitrogen Isotopes (MeSH), Nuclear Magnetic Resonance, Biomolecular (MeSH), Protein Structure, Secondary (MeSH), Toluene (analogs & derivatives), Toluene (chemistry), Toluene (metabolism), Tritium (MeSH), Trypanosoma brucei brucei (metabolism).
MESH :
- chemical , analogs & derivatives : Toluene.
- chemical , chemistry : Glutaredoxins, Toluene.
- chemical , metabolism : Toluene.
- chemical : Carbon Isotopes, Nitrogen Isotopes, Tritium.
- metabolism : Cytosol, Trypanosoma brucei brucei.
- Amino Acid Sequence, Catalytic Domain, Humans, Nuclear Magnetic Resonance, Biomolecular, Protein Structure, Secondary.

Abstract

Trypanosomatids are parasites responsible for several tropical and subtropical diseases, such as Chaga's disease, sleeping sickness and Leishmaniasis. In contrast to the mammalian host, the thiol-redox metabolism of these pathogens depends on trypanothione [bis-glutathionylspermidine, T(SH)2] instead of glutathione (GSH) providing a set of lineage-specific proteins as drug target candidates. Glutaredoxins (Grx) are ubiquitous small thiol-disulfide oxidoreductases that belong to the thioredoxin-fold family. They play a central role in redox homeostasis and iron sulfur-cluster biogenesis. Each species, including trypanosomes, possesses its own set of isoforms distributed in different subcellular compartments. The genome of trypanosomatids encodes for two class I (dithiolic) Grxs named 2-C-Grx1 and 2-C-Grx2. Both proteins were shown to efficiently reduce different disulfides at the expenses of T(SH)2 using a mechanism that involves the two cysteines in the active site. Moreover, the cytosolic Trypanosoma brucei 2-C-Grx1 but not the mitochondrial 2-C-Grx2 was able to coordinate an iron-sulfur cluster with T(SH)2 or GSH as ligand. As a first step to unravel the structural basis for the specificity observed in the trypanosomal glutaredoxins, we present here the NMR resonance assignment of 2-C-Grx1 from the parasite T. brucei brucei.

DOI: 10.1007/s12104-015-9643-x
PubMed: 26386962

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000500
to stream Main, to step Curation: 000500

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Carbon Isotopes (MeSH)</term>
<term>Catalytic Domain (MeSH)</term>
<term>Cytosol (metabolism)</term>
<term>Glutaredoxins (chemistry)</term>
<term>Humans (MeSH)</term>
<term>Nitrogen Isotopes (MeSH)</term>
<term>Nuclear Magnetic Resonance, Biomolecular (MeSH)</term>
<term>Protein Structure, Secondary (MeSH)</term>
<term>Toluene (analogs & derivatives)</term>
<term>Toluene (chemistry)</term>
<term>Toluene (metabolism)</term>
<term>Tritium (MeSH)</term>
<term>Trypanosoma brucei brucei (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Cytosol (métabolisme)</term>
<term>Domaine catalytique (MeSH)</term>
<term>Glutarédoxines (composition chimique)</term>
<term>Humains (MeSH)</term>
<term>Isotopes de l'azote (MeSH)</term>
<term>Isotopes du carbone (MeSH)</term>
<term>Résonance magnétique nucléaire biomoléculaire (MeSH)</term>
<term>Structure secondaire des protéines (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Toluène (analogues et dérivés)</term>
<term>Toluène (composition chimique)</term>
<term>Toluène (métabolisme)</term>
<term>Tritium (MeSH)</term>
<term>Trypanosoma brucei brucei (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Toluene</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutaredoxins</term>
<term>Toluene</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Toluene</term>
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<term>Nitrogen Isotopes</term>
<term>Tritium</term>
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<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Toluène</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glutarédoxines</term>
<term>Toluène</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cytosol</term>
<term>Trypanosoma brucei brucei</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytosol</term>
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<term>Isotopes de l'azote</term>
<term>Isotopes du carbone</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
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<front><div type="abstract" xml:lang="en">Trypanosomatids are parasites responsible for several tropical and subtropical diseases, such as Chaga's disease, sleeping sickness and Leishmaniasis. In contrast to the mammalian host, the thiol-redox metabolism of these pathogens depends on trypanothione [bis-glutathionylspermidine, T(SH)2] instead of glutathione (GSH) providing a set of lineage-specific proteins as drug target candidates. Glutaredoxins (Grx) are ubiquitous small thiol-disulfide oxidoreductases that belong to the thioredoxin-fold family. They play a central role in redox homeostasis and iron sulfur-cluster biogenesis. Each species, including trypanosomes, possesses its own set of isoforms distributed in different subcellular compartments. The genome of trypanosomatids encodes for two class I (dithiolic) Grxs named 2-C-Grx1 and 2-C-Grx2. Both proteins were shown to efficiently reduce different disulfides at the expenses of T(SH)2 using a mechanism that involves the two cysteines in the active site. Moreover, the cytosolic Trypanosoma brucei 2-C-Grx1 but not the mitochondrial 2-C-Grx2 was able to coordinate an iron-sulfur cluster with T(SH)2 or GSH as ligand. As a first step to unravel the structural basis for the specificity observed in the trypanosomal glutaredoxins, we present here the NMR resonance assignment of 2-C-Grx1 from the parasite T. brucei brucei. </div>
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<ArticleTitle>1H, 13C and 15N resonance assignment of the cytosolic dithiol glutaredoxin 1 from the pathogen Trypanosoma brucei.</ArticleTitle>
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<Abstract><AbstractText>Trypanosomatids are parasites responsible for several tropical and subtropical diseases, such as Chaga's disease, sleeping sickness and Leishmaniasis. In contrast to the mammalian host, the thiol-redox metabolism of these pathogens depends on trypanothione [bis-glutathionylspermidine, T(SH)2] instead of glutathione (GSH) providing a set of lineage-specific proteins as drug target candidates. Glutaredoxins (Grx) are ubiquitous small thiol-disulfide oxidoreductases that belong to the thioredoxin-fold family. They play a central role in redox homeostasis and iron sulfur-cluster biogenesis. Each species, including trypanosomes, possesses its own set of isoforms distributed in different subcellular compartments. The genome of trypanosomatids encodes for two class I (dithiolic) Grxs named 2-C-Grx1 and 2-C-Grx2. Both proteins were shown to efficiently reduce different disulfides at the expenses of T(SH)2 using a mechanism that involves the two cysteines in the active site. Moreover, the cytosolic Trypanosoma brucei 2-C-Grx1 but not the mitochondrial 2-C-Grx2 was able to coordinate an iron-sulfur cluster with T(SH)2 or GSH as ligand. As a first step to unravel the structural basis for the specificity observed in the trypanosomal glutaredoxins, we present here the NMR resonance assignment of 2-C-Grx1 from the parasite T. brucei brucei. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stefani</LastName>
<ForeName>Monica</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Chemical Sciences, Università degli Studi di Padova, Via Marzolo 1, 35131, Padova, Italy.</Affiliation>
</AffiliationInfo>
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<ForeName>Mattia</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Chemical Sciences, Università degli Studi di Padova, Via Marzolo 1, 35131, Padova, Italy.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Molecular Modeling Section (MMS), Department of Pharmaceutical and Pharmacological Sciences, Università degli Studi di Padova, Via Marzolo 5, 35131, Padova, Italy.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Manta</LastName>
<ForeName>Bruno</ForeName>
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<AffiliationInfo><Affiliation>Laboratory Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.</Affiliation>
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<AffiliationInfo><Affiliation>Laboratorio de Fisicoquímica Biológica, Facultad de Ciencias, Instituto de Química Biológica, Universidad de la República, Igua 4225, 11400, Montevideo, Uruguay.</Affiliation>
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<AffiliationInfo><Affiliation>Department of Chemical Sciences, Università degli Studi di Padova, Via Marzolo 1, 35131, Padova, Italy.</Affiliation>
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<ForeName>Marcelo</ForeName>
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<AffiliationInfo><Affiliation>Laboratory Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.</Affiliation>
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<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Chemical Sciences, Università degli Studi di Padova, Via Marzolo 1, 35131, Padova, Italy. massimo.bellanda@unipd.it.</Affiliation>
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<Month>09</Month>
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<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Biomol NMR Assign</MedlineTA>
<NlmUniqueID>101472371</NlmUniqueID>
<ISSNLinking>1874-270X</ISSNLinking>
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<affiliations><list><country><li>Allemagne</li>
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<tree><country name="Italie"><region name="Vénétie"><name sortKey="Stefani, Monica" sort="Stefani, Monica" uniqKey="Stefani M" first="Monica" last="Stefani">Monica Stefani</name>
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	Serveur d'exploration sur la glutarédoxine
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Serveur d'exploration sur la glutarédoxine

1H, 13C and 15N resonance assignment of the cytosolic dithiol glutaredoxin 1 from the pathogen Trypanosoma brucei.

1H, 13C and 15N resonance assignment of the cytosolic dithiol glutaredoxin 1 from the pathogen Trypanosoma brucei.

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